WebJun 24, 2024 · Bioworld. AP0063. MTT assay. We detected the proliferation of HO-8910 and OVCAR-3 cells by MTT assay. First, the cells were seeded to 96-well plates and incubated. After cells were digested by trypsin, complete medium was resuspended into cell suspension. The cell density was 2000 cells/well and inoculated to 96-well plates (100 … WebGAPDH (Bioworld, AP0063), anti-phospho-PERK (sc-32577, Santa Cruz), anti-PERK (sc-13073, Santa Cruz), anti-TGF-β(Abcam, ab92486), anti-α-SMA(Abcam, ab32575), anti-Collagen I (Abcam, ab34710) and anti-Collagen III(Abcam, ab7778) were used for Western blotting experiments. Gel Imaging System (Tanon) and
Double Knockdown of PHD1 and Keap1 Attenuated Hypoxia …
WebIn this experiment, we used small hairpin RNA (shRNA) to interfere with the intracellular oxygen sensor—prolyl hydroxylase 1 (PHD1) and the intracellular oxidative stress... Webrabbit anti-HSP90AA1 (Bioworld, BS1181), rabbit anti-HSPA1A (Bioworld, BS6446), rabbit anti-PIM1 (Abcam, ab75776), rabbit anti-AKT1 (Bioworld, BS1978), rabbit anti-GAPDH … eal professional development
Ecust004 suppresses breast cancer cell growth DDDT
WebDec 4, 2015 · Antibodies: Anti-Tgfbr2 (Abgent, AP17322b), anti-GAPDH (Bioworld, AP0063), and goat anti-rabbit IgG-HRP (Bioworld, BS13278) were used. The Animal Total RNA Fast Extraction Kit (Shanghai Generay Biotech Co., Ltd, China) was used to perform RNA extractions. A RevertAid First Strand complementary DNA (cDNA) Synthesis Kit … WebGAPDH Monoclonal antibody 60004-1-Ig is a primary antibody from Proteintech 7510 Citations Host: Mouse Clonality: Monoclonal (1E6D9) Validations: Knockdown Applications: WB, IHC and 8 more Reactivity: Homo sapiens (Human), Mus musculus (House mouse) and 36 more GAPDH (14C10) Rabbit mAb 2118 is a primary antibody from Cell Signaling … WebGAPDH 37 1:3000 Rabbit Bioworld AP0063 MTT assay First, PANC-1 and SW1990 cells were trypsinized, com-pletely suspended and counted. ˙e cell density was 2000 . W et al. Cancer Cell Int P 3 10 cells/well and inoculated to 96-well plate(µ) (ning, Corning, NT, USA#)night, and the cells were repeate–s in each group. 5˚mg/ ... e alphabet in french