WebAug 18, 2024 · Instruction for lysis liquid II. Step 1, shake to mix buffer WA with meat sample, vortex 15 s, add 20 μl Proteinase K and shake for 10 s, water bath at 55 °C for 10 min; Step 2, vortex 30 s, centrifuge at 10000 g for 2 min, and transfer the supernatant 100 μl to a new centrifuge tube. Step 3, add 100 μl deionized water to supernatant and ... WebDirectPCR Lysis Reagent (Cell) is Available in Stock. Size: 100 mL. Lyse tails, Incubate for 45 min at 85°C, PCR genotyping with 1 μl lysates. DirectPCR® DNA Extraction System is a single-tube system for rapid preparation of DNA from mouse tails, ear pieces, yolk sac, and culture cells. The patent-pending components developed by scientists at Viagen Biotech …
DirectPCR Lysis Reagent (Mouse Tail) 50ml 101-T
WebBrief procedure 1. Lyse tails in DirectPCR® Lysis Reagent. 2. Incubate for 45 min at 85°C. 3. PCR genotyping with 1 μl lysates. Detailed protocols: Tail, Ear, Yolk Sac, and Cultured cells. DirectPCR® system offers advantages over conventional protocols that include: · Time saving: Less hands-on time. Crude tail lysates for PCR. WebBrief procedure 1. Lyse tails in DirectPCR® Lysis Reagent. 2. Incubate for 45 min at 85°C. 3. PCR genotyping with 1 μl lysates. Detailed protocols: Tail, Ear, Yolk Sac, and Cultured cells. DirectPCR® system offers … dojrp lspd skins
WebDirectPCR Lysis Reagents Genotyping without DNA isolation Description: Viagen Biotech DirectPCR Lysis Reagents are a full line of single-tube systems for rapid, convenient, and reliable preparation of DNA from mouse tails, ears, yolk sacs, and culture cells. WebDescription: Certain compounds in animal tissues inhibit PCR reactions. DirectPCR Lysis Reagents (Patent Pending) contain inhibitors of these PCR inhibitors. Therefore, DNA … WebApr 25, 2008 · Viagen DirectPCR Lysis Reagent is a buffer used to lyse animal tissue and release the DNA for future applications, such as PCR. It promises to contain inhibitors of … purple 10k