WebMay 20, 2024 · Background: Eukaryotic elongation factor 2 kinase (eEF2K) regulates the elongation stage of protein synthesis by phosphorylating eEF2, a process related to various diseases including cancer and cardiovascular and neurodegenerative diseases. In this study, we describe the identification of novel eEF2K inhibitors using high-throughput screening … WebJan 24, 2014 · Using this high-throughput assay, we screened more than 7000 compounds and discovered additional small molecules that inhibit the ATM kinase and further validated these hits by secondary assays. Introduction
High-Throughput Screens for eEF-2 Kinase - SAGE …
WebFeb 14, 2024 · To identify kinases that would regulate brown adipocyte differentiation and formation, we developed a high-content and high-throughput in vitro image-based screening system that combined two experimental approaches (fig. S1A). WebIntroduced, optimized and conducted an in vitro high-throughput drug screen assay for compounds with activity against liver stage malaria. Discovered and confirmed several compounds active against ... earnyour rainbow
Medicines Free Full-Text Discovery of Novel eEF2K Inhibitors …
WebSep 18, 2024 · A high-throughput screen (kinase reaction performed at room temperature) revealed the compound TCMDS-135051 as a highly selective and specific Pf CLK3 kinase inhibitor. This was confirmed in vivo by the finding that parasite strains that developed resistance against TCMDS-135051 acquired mutations in Pf CLK3. WebThese yeast-based methods range from HTP screens to identify novel hits against promising parasite kinase targets to the identification of potential antiparasitic kinase inhibitors extracted from databases of yeast chemical genetic screens. Keywords: Drug discovery; Drug screening; High-throughput; Kinase inhibitors; Neglected diseases; Yeast. WebOct 26, 2015 · High-Throughput Screens for eEF-2 Kinase. Article. Sep 2013; Ramakrishna Edupuganti; Ashwini K Devkota; Mangalika Warthaka; Kevin Dalby; eEF-2 kinase is a potential therapeutic target for breast ... earn your living creators