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WebJan 25, 2024 · The qPCR reaction system was composed of 3 μl of cDNA, 2.5 μl of 10 × PCR buffer (Mg 2+, TaKaRa Cat no.: 9151A), 2 μl of dNTP mix (TaKaRa Cat no.: 4030), 0.2 μl of TaqHS enzyme (TaKaRa Cat no.: R007WZ), 0.4 μmol/L of each of the primers, 10 μmol/L of probe, and ddH 2 O up to 25 μl. The thermocycling steps was 95 °C for 3 min, … twt inc
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WebApr 9, 2024 · Recurrent pregnancy loss (RPL) occurs in approximately 5% of clinically identified pregnancies. Determining the cause of RPL is essential. Genetic testing, accompanied by an evidence-based workup, is the well-accepted process for evaluating RPL; however, current genetic tests have limitations in clinical practice. We, thus, … Webwere amplified from mouse cDNA by PCR using Ex TaqHS (TaKaRa, Osaka, Japan). Primer sequences for amplifying the MAFbx/atrogin-13-UTRwere5-TCTCTAGAATCCCAG CAC ACG ACA ACA CTT C-3 (forward) and 5-CTC TCG AGGACTCCGTTTCCATGGCTGAC-3 (reverse).Primer sequences for amplifying the … WebNov 4, 2011 · The TaqMan MicroRNA reverse transcription kit and TaqMan MicroRNA assays (Applied Biosystems, Foster City, CA) were used according to the manufacturer's … tamara lich bail hearing today live